High Efficiency protein delivery

Engineered protein transmembrane domain (PTD) The direct introduction of protein into cells is a critical step in studying protein function.  Unlike DNA transfection, in which the transcriptionally active DNA/gene is introduced into living cells where protein is made by cellular machinery, protein delivery does not introduce any exogenous DNA and is independent of the cellular […]

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Enhanced super CMV promoter

Enhanced super CMV promoter RNA polymerase II is the main promoter category driven expression of thousands of genes. Its transcription mechanisms have been widely investigated. It involves many transcription factors and a combinatorial array of cis-regulatory DNA elements. Two parts were well characterized for transcription initiation complexes, the Core promoter and the Co-regulators. The core […]

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PCR cloning -never been easier

PCR cloning kit Cloning is often limited by cost, labor-intensity, and incompatibility of restriction enzyme sites. Traditional in vitro cloning requires a variety of end-joining enzymes (e.g. T4 ligase, toposiomerase, blunt-end joining enzymes) or specific strand-exchange recombinases (e.g. CRE, loxP, Gateway BP, LR recombinase). Generating overhung- or blunt- ends  requires either  preparing vectors by restriction […]

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