GenTarget’s Eco^TM Buster E Coli protein extraction reagent is proprietarily formulated for efficient extraction of active protein from E. coli.  without mechanical lysis. It is nonionic detergent-based lysis (in Tris-HCl, PH7.5), and much better preserve the activity of the proteins than the harsh mechanical lysis, such as grinding or sonication. Compared to other brand lysis reagent, it is more efficient in lysis, and better preserves protein’s activities, enabling to obtain higher yield of active, soluble protein. It is suitable for extracting proteins from wide range of bacterial strains.

Eco^TM Buster protein extraction reagents are provided in Ready-to-use, single solution configuration. If your protein is greater than 75 kDa, use Eco^TM Buster Large Protein Extraction (Cat# EB-L100) which greatly increases the yields for large proteins. For protein smaller than 75 kDa, should use regular Eco^TM Buster Small Protein Extraction (Cat# EB-S100). See product manual.