Pre-made lentiviral particles expressing a fusion target of (CFP-Annexin5). The sequence verified full lengt human Annexin5-ORF (NCBI accesion ID: NM_001154 ) was fusioned in frame with CFP at its N-terminal, expressed under a consitutive suCMV promoter. Fluorescent labelled Annexin 5 (or Annexin V) can be used to detect early apoptotic cells. Annexin 5 interacts strongly and specifically with phosphatidylserine (PS) that exposured from plasma membrane in the early event in apoptosis. Therefore, Annexin 5 can be used to detect apoptosis by targeting for the loss of plasma membrane asymmetry. Labeled annexin V can be applied both in flow cytometry and in light microscopy in living culture culture. Please see product manual for details. Amount: 200ul/vial (1x108 IFU/ml), provided in DMEM medium with 10% FBS and 60ug/ml Polybrene (10x). Cat#: LVP445-C |