Gentarget provides different sets of premade lentivirial particles for human or mouse iPS genes (see iPS particle product page).

This premade lentiviral particles is the single vector method for iPS cell generation. Four mouse iPS factor, c-Myc, Klf4, Oct4 and Sox2 (MKOS) were cloned under the same Tetracycline inducible CMV promoter. All 4 factors were cloned in frame but separated by F2A signal fragment, which result in the expression of four individual factor via "ribosomal skipping" mechanism.

This particle was produced by co-transfection of the chained iPS inducible lentivector with a packaging mix (Cat# HT-pack) into a 293T packaging cells (cat# TLV-C). The pre-made lentiviral particles are VSV-G pseudotyped virus, packaged in 10% FBS medium, and supplied as 500ul/per vial at ~ 1x 10⁶ IFU/ml. The premade repressor lentiviral particles was already pre-mixed with the chained iPSC particles to stop all four iPS factor's expression. The expression of iPS factors only occurs when the Tetracycline or Dox was added into the cell culture. A inducible control GFP particles (also premixed with the repressor) is included in this product.

See product manual (.pdf) for details.

Amounts:

1) mMKOS: 0.5 ml/tube x 2 tubes, at 1 x 10⁶ IFU/ml

2) Inducible control GFP for iPSC: 500ul/tube x 1 tubes, at 1 x 10⁷ IFU/ml

Cat#: LVP359