Cloning is to joint a double stranded DNA fragment with a vector. Traditional cloning utilizes different end-jointing enzymes (such as T4 ligase, Toposiomerase, Blund-end jointing enzymes, or exonuclease plus ligase, etc) or specific strand-exchange recombinases (such as, CRE, loxP, Flp or Gateway BP, LR recombinase) in a In Vitro tube reaction. It requires preparation of vectors to generating overhung- or blunt- ends by restriction enzyme digestion or end polishing steps, or purchasing of specific vectors and recombinases. Thus, cloning is often limited by costs, the labor consuming vector processing and possible incompatible restriction enzyme sites. Gentarget's Eco^TM PCR Cloning Kit utilizes an engineered E Coli strain with enhanced homologous recombination function for an In Vivo jointing reaction. And the strain was pre-processed with different desired vector with low background. And No any tube reactions are required for cloning. Let the E Coli do the job for you In Vivo !. Please see our product page for More details.

GenTarget's proprietary high through cloning technology is a revolutionized and the easiest PCR cloning method to make your E Coli or mammalina expression clones with N- or C-term His / GST tag.  It's key features are:

• The most cost effective and the easiest PCR cloning method, simply add 1ul of PCR insert into provided cells for transformation regardless of the insert's size and concentration;
• No need for the tedious bench works for preparation of vector backbone;
• No need for any enzymes or any tube reactions;
• Precisely directional cloning of PCR products without any extra protein sequences (except affinity tag);
• Does not matter with any PCR products with or without a 3'-end's -A overhung (the extra -A overhang, if exists, will be removed in cloning step);
• Good for different PCR sizes, from 200bp to 10kb.
• Engineered E Coli and mammalian expression vectors for high protein yields;
• Great for high through-put cloning;