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      Pre-made Lentivirus

      GFP CFP YFP RFP->

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      CRE, Luc & Enzymes->

         Luciferase->

            Firefly Luciferase

            Renilla Luciferase

            Gaussia Luciferase

            Cypridina Luc.

         CRE recombinase

         TetR particles

         lacZ particles

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Luciferase lentiviral particles

Luciferase is a generic term for the class of oxidative enzymes used in bioluminescence and is distinct from a photoprotein. There are a group of enzymes. The most famous one is the firefly luciferase from the firefly Photinus pyralis and the luciferase from Renilla. Some luciferases are naturally secreted such as Gaussia Luciferase, Cypridina Luciferase.

Luciferase is widely used in signal enzymatic assays because of its high sensitivity, high linear dynamical ranges, and high through-put assay adaptation.

Different luciferases demonstrate some useful properties (See table below).

Types

Substrate

Wavelength Peak

Secrated

Sensitivity

Require ATP

Measurement

F-luc     (Firefly Luciferase)

D-Lucferin

562 nm

No

medium

Yes

Peaked at 2 seconds, last for ~10 seconds

G-luc     (Gaussia Luciferase)

coelenterazine

480 nm

Yes

extreme

No

Measure luc activity from supernatnat for living cells. Luc activity is stable at low pH; measure within 30 seconds

C-Luc (Cypridina Luciferase)

cypridina luciferin

465 nm

Yes

extreme

No

Measure luc activity from supernatnat for living cells. Luc activity is inhibited by EDTA;
Long half life at ~ 48 hours.

R-Luc(Renilla Luciferase)

coelenterazine

480 nm

No

medium

No

Peaked at 2 seconds, last for 10 second

Gentarget provides luciferase expression particles containing different fluorescnet markers, antibiotic markers or fluorescent-antibiotic fusion dual markers. The fluorescent marker was expressed either under the same promoter with luciferase (Bicistronical expression) or under different promoter. Please see the vector schemes below for details.  

Please see click into each links below for product information.

 

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