CRE protein, from bacteriophage P1, catalyzes recombination between 34 base pair target sequences, named lox sites. CRE lentiviral particles can catalyze the jointing event In Vivo in the presence of lox sites. Purified CRE enzyme can joint individual plasmids containing lox sites.

Gentarget provides premade, expression ready, CRE lentiviral particles. The expressed CRE contains  the nuclear localization signal (NLS), PKKKRKV, from the SV40 Large T-antigen, at its N-terminal, which allows CRE penetrate nuclear membrane. This nuclear permeant CRE increase the recombination events in vivo.

To satisfy all kinds of needs, GenTarget CRE expression lentivirus was provided with different fluorescent markers, antibiotic markers, or fluorescent-antibiotic fusion dual markers. We also provides CRE, luciferase and fluorescnet protein (GFP/RFP) triple labeled lentivirus. Please see different vector schemes below for vector structures.

Preamde CRE expression particles are provided either in DMEM medium with serum or as purified in PBS solution for in vivo application. Please see each product manual below for details.