The Promoterless lentivector products include several lentiviral expression vectors containing different antibiotic markers or fluorescent-antibiotic fusion dual markers. Each vector contains a MCS (multiple cloning sites) region for easy sub-cloning of any desired "Promoter-Target" expression cassette or any "promoter only" insert (dependent the vector types).

The lentivectors were engineered with several key genetic elements (WPRE, cPPT, RRE and so on, see vector map scheme below) in order to produce high titer expression lentivirus upon co-transfected with a packaging plasmid mixture into 293T cells.

Each vector contains an ampicillin maker and can be propagated in LB medium containing 100ug/ml of ampicillin. Verified full vector sequence was provided in the product manual. Please click into each product for details.

Key Features

• A MCS region contains multiple Restriction Enzyme sites for sub-cloning of "promoter-target" cassette;
• For some vectors (see each vector for details), a common used reporter (GFP, RFP, or luciferase) is pre-installed after the MCS region, which will be expressed under any sub-cloned "promoter";
• A variety of antibiotic selection markers satisfy your choices, and fluorescent fusion dual markers provide a convenient tool for tracing virus performance;
• Engineered lentivector backbone for high titer virus production;
• The lentivectors adapt the modification / mutation in its 3 UTR region (SIN feature), which produces only replication-incompetent lentivirus for better Bio-safety;
• The expression lentivectors are fully compatible with most packaging mixtures in the marker in virus production;

Product contents:

Each vector is provided at  6.0 ug /per vial, lyophilized.