LocLight^TM Living cell imaging lentivirus for sub-cellular localization

Lentivirus is the most easiest and most effective method to deliver genes into all majority mammalian cell types, including non-dividing cells, primary cells. And the delivered genes will be integrated into host cell genome for long-term expression.

Utilizing its proprietary lentiviral vector systems, GenTarget generates the LocLight^TM sub-cellular labeling lentivirus product series. The premade lentivirus contains a well-defined cell organelle targeting signal that fusioned with a Fluorescent protein (GFP, RFP or CFP), expressed under our proprietary suCMV prompter. The fluorescent proteins are non-toxic to cells, do not compromise cell structure or interfere with signal pathway evolvement. Therefore they are great tools for live-cell imaging and for dynamically investigation of sub-cellular signal pathways.

The pre-made LocLight^TM sub-cellular labeling lentivirus do not need any extra additives, or substrates for usage. They are extremely easy to use. By simply adding the lentivirus into mammalian cell culture, the expression of auto-fluorescent protein will be localized to the specific sub-cellular compartments at 1-3 days post the transduction, which can be visualized under microscope with corresponding filter sets.

Particles are provided in 200 ul/per vial in DMEM medium with 10% FBS and 60ug/ml polybrene as ready to use status. The titer is about 1x10⁷ IFU/ml measured by fluorescent positive cell counting. 

Key features:

1. High titer and robust: GenTarget's premade lentiviral particles are best in the class, demonstrating the brightest fluorescent signal, strong transduction efficiency. Each particles was validated in lot by lot basis and its quality is guaranteed.
2. Deliver fluorescent labeled targets into hard transfected cell lines (like primary cells or neuron cells) for long term expression;
3. Very easy to us:  Simply add into your cell culture and visualize the fluorescent at 48-72 hours (No need for any additives, substrates, components , cell washes, or medium changes);
4. Different fluorescent labeled particles for multi-color application when multiple different particles used in the same cells;

GenTarget generates the high quality of LocLight^TM cell organelle labeling lentivirus with different fluorescent markers. Please see the following table for the product availability with the organelle targeting information.