Gentarget Inc’s EcoTM Competent Cells were pre-made for chemical transformation or electroporation. Chemical Transformation efficiencies typically range from 108–109 transformants/μg of pUC19 DNA. Electroporation efficiency is 1010 transformants / per μg of pUC19 DNA. Competent cells are supplied as a pack of 20 x 50 μl/tube aliquots, or in a 96-well format. All competent cells were tested on a lot-to-lot basis to ensure the product performance and quality. See product manual. (.pdf file) (We also provide bulk amount of competent cells as a service project for selected or customized cell types at much discounted prices) To select competent cells: Application | Competent cell selected | Cat# | General cloning | EcoTM_DH5α_Cloning ells | CC01 | Lentivrial or Retroviral cloning | EcoTM_DH5a_Lenti cells | CC02 | General protein expression | EcoTM_BL21(DE3) cells | CC03 | Toxic protein expression | EcoTM_BL21(pLys) cells | CC03p | Extreme toxic protein expression, or get protein expressed out of inclusion body | EcoTM_BL21_Toxic_ cells | CC04 | cDNA library construction | EcoTM_DH10_Electro cells | EC01 | High through-put cloning | 96-well plate competent cells | CC-plate |
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Expression lentiviral particles->
