This particles was produced from our SureTiter lentiviral system. GFP  and RFP  are bicistronically expressed under the same suCMV promoter mediated by  F2A self-processing peptide. A Blasticidin marker was expressed under RSV promoter, allowing the selection of transduced cells for long term expression. GFP was expressed with C-term His tag (Note: RFP is not fusuion with GFP).

Application:

1. Used as  control lentiviral particles to monitor the transduction ability for your cell of interest;
2. Visualize expression efficiency by GFP signal and trasnduction efficiency via RFP signal;
3. Used for making dual fluorescent GFP and RFP stable cell line via Blasticidin selection or flurescent cell sorting;
4. Purify mammalian expressed His-tagged GFP protein; 
5. Others;

See product manual (.pdf) for details.

Amount: 200ul/tube, at 1 x 10⁸ IFU/ml in DMEM medium with 10% FBS, 60ug/ml polybrene. 

Cat#: LVP002