Vector expressed RNAi for gene silencing provides an alternative, convenient method to functional studies in both animal and cell line models. Variety RNAi vectors are commercially available now in the market. Take advantage of our extensive experiences in commerical vector development, GenTarget scientists developed several advanced, easier and unique shRNA cloning kits for making both lentiviral vectors and non-lentiviral vector. 

 

Knockdown of endogenous human P53 in A549 cells by pEco-H1-p53-shRNA-(GFP-Bsd)

Gentarget's shRNA cloning kits are  designed for cloning double-strand DNA encoding a short hairpin RNA. The transcription of shRNA was driven by an tetracycline inducible human H1 promoter, for a controled expression of shRNA in tetracycline repressor cell lines. This Kit provides materials (for 20 cloning reactions) for generation of your own shRNA expressing clones with the following advanced features.

1. Linearized vector and ready for use, no need for the tedious bench works for preparation of vector backbone;
2. Precisely directional cloning of your DNA duplex encoded shRNA structure;
3. Rapid, high efficient cloning with low background (>90% positive rate);
4. Internal transfection efficiency reference: the vector encode a GFP fluorescent protein, allowing real-time monitoring and normalizing the transfection differences;
5. Long-term stable silencing effect: the vector encoded Blasticidin (Bsd) resistance marker which is fusioned with GFP, allowing to generate stable cell lines for long-term knockdown of your target;
6. Inducible knockdown: the vector's human H1 promoter was integrated with two Tet-repressor binding-sites (TRBS), allowing inducible expression of shRNA in Tet-repressor expressing cell lines upon induction by tetracycline.
7. Insert compatible: the same annealed shRNA duplex can be readily cloned into all Ready-to-use linear shRNAvectors to make either lenti shRNA or regualr shRNA vectors.

Please see the shRNAi vector product pages for details.