Pre-made tetracycline inducible lentiviral particles expressing sequence verified CRE recombinase under inducible suCMV promoter. A blasticidin-RFP fusion marker under RSV promoter, allows to sort or select transduced cells for long term expression via RFP signal or via blasticidin antibiotic (Dual markers). RFP signal provides a convenient, realtime monitoring the particles performance. See product manual for details (.pdf). Cre protein, from bacteriophage P1, catalyzes recombination between 34 base pair target sequences, named lox sites. CRE lentiviral particles can catalyze the jointing event In Vivo in the presence of lox sites. Purified CRE enzyme can joint individual plasmids containing lox sites. Amount: 200ul/per vial, at 1 x 107 IFU/ml in DMEM medium with 10% FBS. Cat#: LVP013 |