Utilizing its SureTiter lentiviral system or Inducible lentiviral system, Gentarget produced Ready-To-Use lentiviral particles (LP) for many common used gene markers and enzymes. Each target was fully sequencing verified and natively expressed under our proprietary suCMV promoter (click to see details), or under an enhanced EF1a promoter[1]. 

The suCMV promoter demonstrated the strongest constitutive expression activity. And it can also used for tetraycycline inducible expression because the CMV promoter was engineered containing two copies of repressor binding sequences. Therefore, when the repressor protein (TetR) is present, it binds to CMV promoter, which stop the target transcription / expression. And the expression was induced upon the tetracycline added (which realease TetR from the promoter). So the suCMV is an optional inducible promoter (can be used as either constitutive or inducible expression).

The modified EF1a promoter is non-tissue specific, expressed in all cell types, and does not show the promoter-silence effects in tussue or cells after long-term culture. Our modified EF1a promter is a super strong promoter for constitutive expression, best suite for target expression in all tissue types for long term expression research.

A fluorescent marker was included in the most of our viruses, which provides an easy tool to monitore the virus performance or sort the positive infected cells by fluorescent signal. All viruses also contain a antibiotic selection marker, allowing to select the stably expression cell lines.  The fluorescen marker is expressed either under a different promoter than that for the target, or bicistronically expressed under the same promoter as for target but mediated via a 2A element (by which the fluorescent protein and the target were expressed as indivudual protein, not fusion). Please see vector's schematic structure below.

   

Particles were pseudotyped with VSV-G protein and packaged from 293T cells in either DMEM medium with 10% FBS or purified into PBS solution, provided as 200 μL/vial containing > 1 x 10^7-8  IFU (Infection Function Unit) /ml.

The pre-made LPs are ideal for expression ready gene delivery into the cell lines of your interest, for assays both in vivo or in vitro. They are extremely easy to use, no need for any mixing or addition (no need for any tranfection reagents), simply add certain amount of virus into your cultured cells. You will be able to observe the expression in 72 hours.  

Please see product categories below for each product detail (and see aslo each product manual inside).

1. Luciferase lentiviral particles; 
2. CRE recombinase lentiviral particles;
3. TetR (tetracycline Regulator) lentiviral particles;
4. LacZ (β-Galactosidase) lentiviral particles;
5. β-Lactamase lentiviral particles;
6. SV40 large T antigen lentiviral particles;
7. Other enzyme's lentiviral particles;

Note: All our lentivirus are pre-made, in stock, ready to ship.