Converting fully differentiated mouse or human somatic cells into embryonic-like cells (so called induced Pluripotent Stem Cell: iPSC) has attracted enormous attention in stem cell research. Multiple reports have demonstrated that iPS cells were generated by using a set of transcription factors or stem cell factors that delivered as expression virus or expressed proteins. Although the combination of reprogramming factors may slightly different, the main stem cell factors are: OCT3/4, SOX2, NANOG, LIN28, c-Myc and KLF4.  iPSC holds the promise of curing many human diseases and accelerates the stem cell research.

• Utilizing its Inducible lentiviral vector system (see vector scheme below), Gentarget generate high-titer inducible lentiviral particles for all six human or mouse stem cell factors. Each factor was fully sequencing verified and matched to the CDs in NCBI database (see details from each product). High titer lentiviral particles / supernatant were produced in 293T packaging cells (Cat# TLV-C) in serum-free medium. They are psudotyped with VSV-G glycoprotein.

  

Gentarget provides both human and mouse iPS lentivirus that driven by super CMV promoter with either RFP-Blasticidin or Neomycin  antibiotic marker (see vector scheme above), Those lentivirus can be used for constitutive high expression. However, the same particles can also be used as tetracycline inducible expression only when a repressor regulator protein (TetR) is present. (see How tet inducible works.).

• Gentarget also provide human iPS lentivirus that driven by a constitutive EF1a promoter without any markers (see vector scheme below).

• To symplify the iPS cell generation, an inducible single vector particles is also available. The single vector contains the main four iPS factors, Myc, Klf4, Oct3 and SOX2 (MKOS) which were chained under an inducible EF1a promoter, but expressed as induvidual protein mediated by F2A fragment (see vector schematic map below). 

 

iPS lentiviral particles provide an easy tool to generate your own iPS cells. Gentarget's iPS lentivirus was validated in generation of iPS cell and you can get your iPS cells in as short as in 14 days. Please refer to our Validated iPS cell generation procedures.

Please see details in product pages for each premade iPS particles:

A) Human iPS set (NANOG, OCT4, SOX2, KLF4, Myc, LIN28).

B) Mouse iPS set (NANOG, OCT4, SOX2, KLF4, Myc, LIN28).

C) Other potential iPS factors.

 iPS clone images generated using Gentarget's iPS lentiviral particles:

       

 [ Images above were provided by Dr. G. Wang in Harvard Meical School, Boston, MA 02115.]

       

References:

1. NIH stem cell training program (Link).
2. Masaki Ieda, Ji-Dong Fu, et al. (2010). Direct Reprogramming of Fibroblasts into Functional Cardiomyocytes by Defined Factors. Cell 142, 375-386. 
3. Takahashi, K. and Yamanaka, S. (2006). Induction of pluripotent stem cells from mouse embryonic and adult fibroblast cultures by defined factors. Cell 126, 663-676.
4. Yu, J., Vodyanik, M.A., Smuga-Otto, K., Antosiewicz-Bourget, J., Frane, J.L., Tian, S., Nie, J., Jonsdottir, G.A., Ruotti, V., Stewart, R., Slukvin, I.I., and Thomson, J.A. (2007). Induced pluripotent stem cell lines derived from human somatic cells. Science 318, 1917-1920.
5. Park, I.H., et al., Reprogramming of human somatic cells to pluripotency with defined factors. Nature, 2008. 451(7175): p. 141-6.
6. Shao, L., et al., Generation of iPS cells using defined factors linked via the self-cleaving 2A sequences in a single open reading frame. Cell Res., 2009. 19(3): p. 296-306.