rtTA (reverse tetracycline transcriptional activator) stable cells with different marker
Pre-made stable cell line, derived from Hela cells, constitutively expresses the rtTA-m2 (ABC65845.1) different fluorescent-antibiotic fusion markers. The rtTA-m2 was expressed in high level under the enhanced EF1a promoter. The antibiotic marker or the fluorescent-antibiotic marker is constitutively expressed under the RSV promoter. Please see the cell line genomic integrated expression cassette (below).
This cell line expressed rtTA protein binds to tetracycline operator elements (TetO) in the presence of doxycycline (Dox). Any promoters that embedded with TetO sequences can binds to the rtTA protein when the inducer (Dox or tracycline) is present, which activates the transcription of the promoter’s downstream gene (So called, Tet-On inducible system). Therefore, this cell line can be used for Tetracycline or Dox inducible target expression when applied with target expression vectors (with TetO inducible promoter), or for generation of any inducible target stable cell lines (after introduced a TetOn expression vector).