Luciferases

Luciferase Expression Lentivirus

Luciferase is a generic term for the class of oxidative enzymes used in bioluminescence and is distinct from a photoprotein.  The most well-known luciferases are from the firefly Photinus pyralis and the sea pansy Renilla reniformis. Some luciferases are naturally secreted, such as Gaussia luciferase and Cypridina luciferase. The luciferase signal can be easily detected, and luciferase enzymatic assays are widely used because of their high sensitivity, high linear dynamical ranges, and high through-put assay adaptation. Click Product Manual for details (.pdf).

Different luciferases demonstrate some useful properties (See table below). 

Types

Substrate

Wavelength Peak

Secreted

Sensitivity

Require ATP

Measurement

F-luc (Firefly Luciferase)

D-Lucferin

562 nm

No

medium

Yes

Peaked at 2 seconds, last for ~10 seconds

C-Luc (Cypridina Luciferase)

cypridina luciferin

465 nm

Yes

extreme

No

Measure luc activity from supernatnat for living cells. Luc activity is inhibited by EDTA; Long half life at ~ 48 hours.

R-Luc (Renilla Luciferase)

coelenterazine

480 nm

No

medium

No

Peaked at 2 seconds, last for 10 second

TypesF-luc (Firefly Luciferase)

Substrate – D-Lucferin

Wavelength Peak – 562 nm

Secreted – No

Sensitivity – medium

Require ATP – Yes

Measurement – Peaked at 2 seconds, last for ~10 seconds

TypesC-Luc (Cypridina Luciferase)

Substrate – cypridina luciferin

Wavelength Peak – 465 nm

Secreted – Yes

Sensitivity – extreme

Require ATP – No

Measurement – Measure luc activity from supernatnat for living cells. Luc activity is inhibited by EDTA; Long half life at ~ 48 hours.

TypesR-Luc (Renilla Luciferase)

Substrate – coelenterazine

Wavelength Peak – 480 nm

Secreted – No

Sensitivity – medium

Require ATP – No

Measurement – Peaked at 2 seconds, last for 10 second

GenTarget provides luciferase expression particles containing a wide selection of fluorescent markers, antibiotic markers, and fluorescent-antibiotic fusion dual markers. The different luciferase is expressed under  the optional inducible CMVEF1a or a CAG promoter. The fluorescent markers are expressed either under the same promoter with luciferase (bicistronic expression) or under another promoter,  the Rsv promoter (see lentivector core structure scheme below).

GenTarget’s Luciferase Expression lentiviruses are provided as 200 µl/per vial in two formats:   

  • DMEM medium with 10 % FBS and 60 µg/ml polybrene (10x)
  • PBS solution, which is best for in vivo applications, cell cultures requiring serum-free conditions, or for hard-to-infect cells. 

 Note: Ultra concentrated virus (>= 109 IFU/ml) is available upon request.

Please see click each type of luciferase below for product information. Or click Product manual for details (.pdf).

  • Product Name
    Catalog #
    Price
    Buy Now
  • Cypridina Luciferase (12)
  • Firefly Luciferase (85)
  • Renilla Luciferase (26)