One anti-microRNA lentiviector (5.0 ug) and its ready-to-use anti-miRNA lentivirus (0.5 ml), and One negative control anti-miRNA lenitvector (5.0 ug) and its control lentivirus (0.5 ml).
The anti-miRNA was expressed under an optional Tetracycline inducible human H1 promoter. It can be used as constitutive anti-miRNA expression without any induction, or used as tetracycline inducible expression when the Tet-repressor protein is present in advance. The anti-miRNA lentivector aslo contains contains a RFP-Puromycidin fusion dual marker under Rsv promoter, which allows to select the positive cells by Puromycin antibiotic selection or by cell sorting via RFP signal. See Anti-miRNA lentivirus manual for more details about the anti-miRNA lentivector.
Order will be delivered within 7-10 business days.
Cat#: A-miRNA H1-RP,
Amounts: (1) 0.5 ml of pre-made specific anti-miRNA lentivirus with titer at ~ 1×107 IFU/ml;
(2) 0.5 ml of pre-made negative control anti-miRNA lentivirus with titer at ~ 1×107 IFU/ml;
Note: please provide the miRNA ID during checkout in the “Comments” field, or email us the miRNA target ID or its mature miRNA sequence that you want to inhibit or repress.
Kluiver J, Gibcus JH, Hettinga C, Adema A, et al. (2012) Rapid Generation of MicroRNA Sponges for MicroRNA Inhibition. PLoS ONE 7(1): e29275.