This particles was produced in our tetraycline inducible lentiviral system. It expresses codon optimized BFP under tetracycline inducible TetCMV promoter. A Puromycin marker under the constitutive RSV promoter, allows to select transduced cells via Puromycin antibiotic.
BFP is constitutively expressed in high level without any induction. But optionally, BFP can be used as Tetracycline inducible in the prescence of TetR protein (repressor).
GFP signal can be visualized under fluorescent microscope. The puromycin is constitutively expressed and not affected by Tetracycline induction.
The particles was concentrated and buffer exchanged into PBS. (See Product manual (.pdf).
Amount: 200ul/per vial, at 5 x 107 IFU/ml in PBS.