PCR cloning kit to make high-yield E Coli expression vector for expressing N-term His tagged protein. A T7-based vector was built-in with the cloning cells (please see product manual for vector map and details.). Full vector sequenceing can be download from our website.
- 10 tubes x 50ul/ea of vector built-in, Cloning-Ready cells, for 10 cloning reactions;
- 1 tube x 5ul of positive PCR cloning control;
- Forward and reverse sequencing primers: 15ul/ea at 25ng/ul;
See Product Manual (.pdf) for details.