Premade shRNA lentivirus, knockdown human P53 gene (NM_000546). It contains a GFP-Puromycin dual selection marker. GFP fluorescent signal provide a convenient method to observe the virus transduction under microscope, and the transduced cells can be sorted / selected via either fluorescent signal or Puromycin.
shRNA sequence was designed in human P53 codon (ORF) region, demonstrating greater than 75% knockdown levels for human P53 gene.
This is the concentrated shRNA lentiviurs, provided in PBS solution, used for hard-to-transduced cell types or for serum-free cell culture.
Its matched negative control shRNA lentivirus is: CAT#: H1(shRNA-Ctr)-GP-PBS
Please see Product Manual for more details.
Amount: 200ul/tube at (5 x 10 7 IFU/ml) in PBS