GenTarget's proprietary Fusion in vivo, high through-put, Eco cloning technology (patent pending) is a revolutionized and the easiest PCR cloning method. It utilizes an engineered E Coli strain with enhanced homologous recombination machinery for cloning. A specific vector is already built within the cloning cells for an In Vivo end jointing reaction. A proprietary processing ensure low background and > 90% positive cloning rate.
Simply amplifies your gene of interest with a primer pair that flanked with short homologous arm to the expression vector, then add 1ul of purified PCR into the engineered, Ready-to-use Cloning cells, and immediately proceed to transformed. No need to purchase either the expression vectors, or the competent cells. The optimized E Coli or mammalian expression vector was already built within each kind of cloning cells. You just select the cloning cells having the built-in vector that meed your needs. (Notes: to use your own vector, we can build it into our engineered cloning cells in the service project).
For E Coli expression, the protein is expressed from T7 based or non-T7 based vector (dependent the cloning kit). For expressing toxic protein, please select our basal level self-controlled PCR cloning cells (Cat: IC-1003).
The easiest PCR cloning method also eliminates the labor consuming and sometime troublesome vector preparation procedures as in any other cloning. It does not need any kind of In Vitro tube reaction, such as ligation, Topo jointing or In-fusion reaction, and so on. Let the E Coli do the job for you In Vivo!
Please select clonning kits below, and see details from each product manual.
We provide instant cloning kits (EcoTM PCR cloning kits, see below products) that pre-made with different vectors for E Coli or mammalina expression of N-terminal His-tagged or GST- tagged proteins. We also provide kits that pre-made with fully Gateway-Cloning compatible vectors so that you can make Gateway ENTRY or DEST clones without using any recombinases, like BP or LR clonase.
Key Features:
The most cost effective and the easiest PCR cloning method;
No need buy vector, thus eliminates the tedious bench works for preparing vector backbone;
No need to buy cloning competent cells;
No need for any enzymes or any tube reactions;
Precisely directional cloning of PCR products without any extra amino acids except the affinity tag;
Flexible to add any cleavage site for removal of N-term His-tag if desired;
Works with any PCR products with or without a 3'-end's -A overhung (the extra -A overhang, if exists, will be removed in cloning step);
Good for different PCR sizes, from 200bp to 10kb and the same PCR product can be used to construct multiple different expression vectors;
Engineered E Coli and mammalian expression vectors for high protein yields;
Great for high through-put cloning;
How to select PCR cloning kit:
| Cat# | Product | Applications |
IC-1001 | pEco-T7-nHis PCR cloning kit | E Coli expression of N-term His-tagged protein |
IC-1002 | pEco-suCMV-nHis-(Neo) PCR cloning kit | high-yield mammalian expression of N-term His tagged protein |
IC-1003 | pEco-BAD-nHis PCR cloning kit | E Coli expression of N-term His-tagged toxic protein |
IC-1004 | pEco-T7-nGST PCR cloning kit | E Coli expression of N-term GST-tagged protein |
IC-1005 | pEco-ENTRY PCR cloning kit | Making Gateway Entry clone without using BP clonase |
IC-1006 | pEco-T7-cHis PCR cloning kit | E Coli expression of C-term His-tagged protein |
IC-1007 | pEco-CMV-cHis-(Neo) PCR cloning kit | Mammalian expression of C-term His-tagged protein |
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