All particles in this catagory were generated using Gentarget's tetracycline inducible lentiviral system.  Each human gene was fully sequencing verified, and 100% matched to individual NCBI accesion ID at its CDS region (gene codons). All particles are pre-made, in stock, ready-to-ship, provided as 200ul /per vial (Note: shipped in dry-ice package via next day express).

The human targets were natively expressed under tetracycline inducible suCMV promoter. A blasticidin-RFP fusion marker under RSV promoter, allows to sort or select transduced cells for long term expression via RFP signal or via blasticidin antibiotic (Dual markers).  RFP signal provides a convenient, realtime monitoring the particles performance. See vector scheme below:

All inducible lentiviral particles here can be used as regular constitutive expression. However, when inducible  expression is desired, they can be used as tetracycline inducible expression in the presence of a repressor protein (tetR). As inducible expression, the target expression was first repressed by the repressor protein (TetR, tetracycline binding protein) and induced by adding tetracycline. The presence of TetR can be achieved by co-infected premade TetR lentiviral particles or co-trasnfected with a TetR expression plasmid, or simply by a tetR expressing stable cell line. Please see "FAQ about premade lentiviral particles" for more information. Please see Product manual for product details.

GenTarget also provides the Negative control lentivirus (click this link to see each controls) for establishing the control effects of virus infection upon a given cell line, which validates the specificity of any target expression effects.

Please check the Product List file (.pdf) for all available particles for expression of human gene. You can also search by gene symbol or accession ID in "Search in Product Descriptions" using "Advanced Serach" in home page. If you do  not find the gene of your interest, we can generate the ready-to-use particles for you as a lentiviral service project.

Pre-made over-expression lentivirus are easy to use, simply add ceertain amount of virus into your cell culture and wait for 3 days for the expression peak. Please see "transduction protocols" for the application method.

Please Click the link here for each pre-made over-expression lentivirus.