Expression ready lentiviral particles are most advanced tools for gene delivered into wide range of cell types including primary cells and non-dividing cells. The validated shRNA expression particles constain a shRNA sequence that demonstrated greater than 75% knockdown to its specific target. The knockdown validation tests were carried out by a reporter assay where the specific target was fusioned with lacZ or luciferase reporter.

Applications for validated shRNA lentiviral particles:

1. Deliver expression knockdown into hard-to-transfected cell types (such as neuron cells) without using any transfection reagents;
2. Create knockdwon stable cell lines for long-term study;
3. Knockdown target in primary cells, or drug-arrested cells;
4. Others;

Using our Inducible lentiviral shRNA vectors, GenTarget produced shRNA expression lentiviral particles that guarantee knockdown the specific target (>75%). Particles can be used as constitutuve high expression, or optionally, as Tetracycline inducible expression;

 

Each vial of lentiviral particle contains highly transducible lentivirus of >1 x 10e7 IFU/ml x 200ul. (Our titers are validated by true infectious functional units via fluorescent cells). They are easy to use, no need for any mixing or addition, simply add certain amount of virus into your cultured cells. You will be able to observe the shRNA expression in ~ 72 hours.  The shRNA expression can be visulized by the fluorescent signal under microscopy so you know virus' performance in real-time.  Seeing is believeing!  Pease see FAQ about pre-made lentiviral particles for general questions. Please see the following link for general guildline for viarl transduction:

Transduction protocols for adhesive and suspension cells. 

Features of premade shRNA lentiral particles:

1. Particles were privided in serum free medium. 
2. Easy and Ready-to-use: directly added into cells. No need any lipids or transfection reagents,
3. Particles can be used for constitutive high gene expression, or as Tet inducible expression in the presence of TetR (a repressor protein).
4. Safe to use: Self-inactivation to prevent replication of the viron,
5. High true titer: >1 x 10⁷ IFU/ml or higher,
6. Easy and realtime monitoring of the target's expression via fluorescent signal,

Please click each product to see its product manual for details. We aslo provide  Lentiviral services to generate your specific shRNA lentiviral particles or your gene expression lentiviral. Please contact us for a service quote!