Expression ready lentiviral particles are most advanced tools for gene delivered into wide range of cell types including primary cells and non-dividing cells. The validated shRNA expression particles contains a shRNA hairpin insert that demonstrated greater than 75~95% knockdown to its specific target. The knockdown validation tests were measured by a reporter assay where the specific target was fusioned with lacZ or luciferase reporter. The knockdown levels were reflected by the decreases of lacZ or luciferase activity.

Applications for validated shRNA lentiviral particles:

1. Deliver expression knockdown into hard-to-transfected cell types (such as neuron cells) without using any transfection reagents;
2. Create knockdwon stable cell lines for long-term study;
3. Knockdown target in primary cells, or drug-arrested cells;
4. Others;

Using our Inducible lentiviral shRNA vectors, GenTarget produced shRNA expression lentiviral particles that guarantee knockdown the specific target. Particles can be used as constitutuve knockdown, or optionally, as Tetracycline inducible knockdown;

 

Each vial of lentiviral particle contains highly transducible lentivirus of >1 x 10e7 IFU/ml x 200ul. (Our titers are validated by true infectious functional units via fluorescent cells). They are easy to use, no need for any mixing or addition, simply add certain amount of virus into your cultured cells. You will be able to observe the shRNA expression in ~ 72 hours.  The shRNA expression can be visulized by the fluorescent signal under microscopy so you know virus' performance in real-time.  Seeing is believeing!  Pease see FAQ about pre-made lentiviral particles for general questions. Please see the following link for general guildline for viarl transduction:

Transduction protocols for adhesive and suspension cells. 

Features of premade shRNA lentiral particles:

1. Easy to-use: directly added into cells. No need any lipids or transfection reagents,
2. Optional inducible shRNA expression: Particles can be used for constitutive knockdown, or as Tet inducible shRNA expression in the presence of TetR (a repressor protein).
3. Safe to use: Self-inactivation to prevent replication of the viron,
4. High true titer: >1 x 10⁷ IFU/ml or higher,
5. Dual selection marker: Easy and realtime monitoring of the target's expression via fluorescent signal and positive transduced cells can be selection via an antibiotic, puromycin or blasticidin.

We aslo provide  Lentiviral services to generate your specific shRNA lentiviral particles or your gene expression lentiviral. Please contact us for a service quote!

Validated shRNA lentivirus silences a specific target with 75%~95% knockdown levels (dependent upon cell types). Particles also contains a "fluorescent-antibiotic" fusion dual marker (GFP or RFP fusion with puromycin or blasticidin). The transduced postive knockdown cells can be slected via a Fluorescent signal or via an antitioic. Please see each product manul for more details.