Using our unique protocol, GenTarget provide services in antibody engineering and expression. We will determine the Variable region and the secreted leader sequences of the light-chain and heavy-chain from the hybrodoma cell line you provided, for determination of isotype of the monoclonal antibodies of the hybridoma. Moreover, we then can construct those variable sequences into our antibody expression vectors (in either one vector system or two vector system), assembling with different species' constant regions as human, mouse or rabbit. Those constant regions are codon optimized for maximum expression in mammalian expression. The expression leader was engineered for maximum antibody secretion. Upon requested, we can construct any kinds of chimerical vectors for antibody humanization and engineering.  Please contact us for quote about this service.

What we offer:

• Isolate the total RNA and mRNA from collected hybridoma cell pellet;
• Determine the variable and leaser's sequences for antibody's light-chain and heavy-chain;
• Clone the determined variable into our mammalian expression vectors;
• Pioneer expression the antibody in suspension 293 cells;
• Stable antibody expression cell line construction;

Why use GenTarget's antibody cloning / expression service:

• An unique protocol for variable region sequence determination;
• Our engineered CMV promoter give extreme high expression;
• Our engineered leader give high secreted antibody expression so that antibody can purified directly from the media;
• Expressed antibody can be humanized, which has less immunogenic to human;
• Fast turnaround time, excellent cloning experience with the best price;
• Upon requested, Vector can use either Glutamine-synthesis gene or Blasticidin resistant gene or any other you selected marker for easier stable cell line construction;