GenTarget has extensive experiences in "Computer designed soup PCR " based Gene Synthesis. We synthesized many "high value" cDNAs that hard to be isolated from our cDNA library collection. Our optimized protocols allow us to synthesize genes having high GC content and high homologues repeats. For examples, We had successfully synthesized the codon optimized, IgG heavy chains and light chains of human, mouse and rabbit that have highly homologous repeats. Now we'd like to offer this economical service to emphases our commitment in better serving our customers. Our flat price is as low as $0.70/each base.

Service Description:

• Provide customers the full sequence-verified genes that cloned in either Gateway entry vector (default) or any Gateway expression vectors (upon customer's requests with extra charge);
• Free Codon optimization for E Coli, mouse or Mammalian expression;
• Synthesis Deliverable:

1. 3-5 μg of purified plasmid DNA;
2. Bacterial strain containing the plasmid in 20% Glycerol stock media;
3. Final report with plasmid sequence containing the synthesized gene sequence;

Why use GenTarget's gene-synthesis service:

• Less downstream works for customers: the final gene was cloned in fully Gateway compatible Entry Clone so that it can readily be transferred into any Gateway expression clones that customer desired (If requested, Gateway expression cloning will carried out with minimal extra cost);
• You get exact sequence you needed: we guarantee the sequence delivered will exactly match the sequence ordered;
• Cost effective: our competitive price is at as low as $0.70/per bp (minimal $400 order);
• Very fast turnaround time: synthesis completed in 1~2 week for <1.5kb gene, and 2~3 weeks for >1.5kb;
• IP and Confidentiality: You own full intellectual prperty rights to the gene delivered. Our services are purely based on a "fee for service" model. We will not disclose any service information to others without your permission.

How to order:

1. Download this Gene-synthesis-order-form (word .doc file);
2. Paste your sequence, and fill out this order form with a formal PO# or credit card. The sequence can be entered as DNA base sequence as well as protein amino acid sequence. If the sequence entered as protein sequence, select the codon species for sequence codon optimization, mammalian Coli. mouse or human;
3. Email  or Fax to GenTarget the Gene-synthesis-order-form ;
4. GenTarget send back an email with the final Quote for a confirmation;
5. Upon approved, GenTarget design and order the Oligonucleotide, and  proceed to the assembling, cloning and sequence verification