E Coli expression and purification:

GenTarget's proprietary Fusion in vivo,  PCR cloning technology provide us an edge for the easiest cloning of your target into our optimized expression vectors. Our auto-induction medium provide us induction worry-free protocol for the expression. In the case of that the expressed protein is very toxic or mainly expressed in inclusion body, we will use our self-tight-controlled cloning cells for your target. The expressed protein (with His-tag) will be purified by affinity column. Please contact us for a service quote.

Mammalian suspension expression and purification:

Utilizing GeTarget's advanced lentiviral vector system, we will clone your target into our lentiviral vector with a His tag, generate expression lentiviral particles, and transduce them into mammalian suspension cells for expression. Then under the antibiotic selectin pressure,  only transduced cells grow and expressed the target protein. It is an controlled semi-stable cell line expression system, demonstrating high protein expression levels without really building a stable cell line. The beauty of using lentiviral system is that lentiviral particles give highest transduction rate in suspension cells that can easily generate a large population of target expressing cells. The is a great way to obtain mammalian expressed protein with very low costs! The expressed proteins will be purified by affinity column (His-tag). Please contact us  for a service quote.