Mammalian stable cell line creation

GenTarget's stable cell line service can build your cell line at a very cost effective way. Using our proprietary SureTiter^TM lentiviral vector developed in house, We will install your target gene into our lenti vector, generate the lentivirus particle, sequentially infect the cell line you desired, and select the clones constantly expressing your gene at high level. Our proprietary suCMV promoter has the strongest expression efficiency, will provide extremely high level of expression for your target. We will work with you to ensure that the cell line meets your requirements. Our unique technology ensures a fast turn around time with less than 2 month for each cell line creation. Cell lines are shipped as cryopreserved vials after validating the target gene's integration. You will get the best quality and best price.

Service details:

• Construct your gene into our lentiviral vector;
• Generate lentivirus;
• Transduce the cell line of your interest;
• Select the stably transduced, high expression cells;
• Validation the genomic integration of gene via genomic PCR;
• Validation the high-expression clone by Western Blot (if applicable);
• Deliverable: Two cryogenic preserved vials of stable cells and final report;

What's the advantages for using lentivirus to generate stable cells?

To make stable cell line, taeget has to be intergraded into host cells' genome for a stable, constitutive expression. Randomly integration (that such as by transfection) demonstrates large variety expression dependent upon the transcription levels at integration sites. And random integration often resulted in the independent integration between the target and the selection marker, which lead to large scale of screening works for selecting the positive clone (resistant with high expression). In general, less than 10% resistant clones express the transgene. In contrast, lentivrius transduction has a tendency to integrate in high transcription level sites (hot-spots) with a full virus genome. Besides, engineered lentiviral transfer vector that embedded a matrix-attachment region (MARs) sequence may provide position-independent transgene expressions. Compared to conventionally stable cell line construction, lentivirus has much higher positive clone rate and target is always co-exist with the selection marker, thus substantially reduces the cost, labor and time in selection of high-level expression stable clones.

Why use GenTarget's stable cell line service:

• Engineered in house lentiviral vector for high efficient gene integration into cell genome with extremely low false positive clones;
• Self-inactivating (safe to use) and high-titer lentiviral particles can delivery your gene into a wide range of cell lines including non-dividing, primary or stem cells;
• Target protein expression can be traced and monitored by a fluorescent image, suitable for sorting or selecting the integrated cells (noticed: the fluorescent protein is not fuioned with your target);
• Our Experts with years of experience in lentiviral cloning and expression;
• Fast turnaround time with the best price;

How to order:

• Please fill out this Service Request Form or simply Contact Us  or Email us for a quote

Please also check our catalog products for pre-made Stable Cell Lines!