Lentivirus Service

Gentarget-lentivirus-services

 

Top quality, Best price and Fastest turnarund time, with >15 year lentivirus experience!

 

(1) shRNA lentiviral services: 

We construct shRNA target knockdown lentivirus by either using your validated shRNAs or by our designed shRNA sequences for your genes of interest. We then generate 0.5 ml of ready-to-use shRNA lentivirus as well as the constructed shRNA lentivectors.

  • You choose the shRNA lentivectors (see the vector map scheme below)
  • We deliver the sequence verified shRNA lentivector and ready-to-use high-titer shRNA lentivirus to you.
  • We provide you with free negative control shRNA (Null-Ctr) lentiviral particles.

Note: Knockdown validation is not included, but if you order 3 shRNAs for one target, we guarantee either at least one shRNA showing >75% knockdown or a knockdown level of >75% by using the pooled three shRNA lentiviruses.

Price $1400.00 USD for Four shRNAs (three target shRNA plus one negative control shRNA) lentiviruses (0.5ml/each) and vectors (5ug/each) with guaranteed knockdown.

Timeline: 1 ~ 2 weeks.

GenTarget_shRNA_Lentivector_Schemes

Citations: Huanhuan Joyce Chen (et al), J Clin Invest. 2012;122(9):3184-3196. Chemokine 25-induced signaling suppresses colon cancer invasion and metastasis.

(2) Over-Expression lentiviral services:  

We construct the lentivector and produce the ready-to-use over-expression lentivirus for your desired target.  

  • You choose the expression lentivector (see the vector map scheme below) ;
  • We sub-clone your target into our lentivectors;
  • We deliver the high titer, ready-to-use lentivirus to you ;

You have the following options with this service

  • Promoters:  suCMV, inducible CMV, EF1a, or CAG
  • Selection markers: Blasticidin or Puromycin or antibiotic-fluorescent fusion dual marker
  • Fluorescent tag-target fusion
  • Any Tags at either the N- or C-terminus
  • A N- terminus fluorescent fusion marker (GFP, RFP or CFP);
  • Or bicistronic expression where target and a fluorescent marker expressed under the same promoter as two separate proteins;

Note: Our optional inducible CMV promoter can be used for either constitutive or inducible expression.

          Schematic representation of gene expression Lentiviral vectors

GenTarget_cDNA_clone_expressoin_Lentivector_Schemes

GenTarget_Biscistronic_Lentivector_schemes

GenTarget_Fluorescent_Fusion_Expression_Lentivectors

Citations: Stancy Joseph, et al. PLoS ONE 7(7): e42159 (2012). ARID3B Induces Tumor Necrosis Factor Alpha Mediated Apoptosis While a Novel ARID3B Splice Form Does Not Induce Cell Death.

Adnan Dibas, et al. IOVS, May 2012, Vol. 53, No. 6. Fluorescent Protein–Labeled Glucocorticoid Receptor alpha Isoform Trafficking in Cultured Human Trabecular Meshwork Cells

(3) Signal reporter lentivirus services: 

We construct the lentivector by sub-clone your desired promoter into our promoter-less lentivector, and deliver you the ready-to-use lentivirus.  

  • You choose the signal reporter type and selection marker(see the vector map scheme below) ;
  • We sub-clone your promoter sequence into our lentivectors;
  • We deliver the high titer, ready-to-use lentivirus to you ; 

GenTarget_TRE_Signal_Transduction_Pathway_lentivectors

  (4) miRNA expression and anti-miRNA lentivirus services:

  • miRNA expression

    We construct an expression lentivector and produce ready-to-use lentivirus for the precursor microRNA expression of any human or mouse miRNA  listed in miRBase database.

  • Anit-miRNA: 

    We construct an anti-miRNA lentivector and produce ready-to-use lentivirus for inhibition of any miRNA listed in the miRBase database. You simply select the miRNA you want to inhibit, and we deliver the order within 7-10 business days.  

    Each order will contain the following items: 

    • One specific anti-miRNA lentivector (5.0 ug),
    • 0.5 ml of pre-made anti-miRNA lentivirus (specific to the desired miRNA) and 0.5 ml of negative control anti-miRNA lentivirus

Gentarget_anti-miRNA_Lentivector_Maps

Why use Gentarget’s Lentivirus Services: 

  • Take advantage of Gentarget’s extensive hand-on experiences in cloning and lentiviral system, saving you the time of performing difficult lentiviral experiments
  • We use the strongest prompter for the highest protein expression levels in mammalian cells
  • Fluorescent protein monitoring ensures high virus titer
  • Fast turnaround time Best price and
  • Best quality in the class

(5) CRISPR / Cas9 lentivirus services:

Gentarget provide pre-made CAS9 expression lentivirus with different antibiotic / fluorescent markers, and design and construct gene specific guild RNA lentivectors (gRNA lentivectors), then produce ready-to-use targeting gRNA lentivirus for gene knockout, and optionally synthesize the single-strand or double-stranded loci specific repair insert or knock-in cassette for gene editing.  See gRNA lentivector core map scheme below. You will receive (1) expression ready humanized CRISPR/Cas9 lentivirus, (2) a set of ready-to-use target gRNNA lentivirus, (3) when requested, the repair / knock-in / mutation / of DNA cassette or single strand repair DNA oligo.

GenTarget_CRISPR-Lentivector_scheme

How to order: 

Simply Contact us by email or Phone for a service quote. Or Download and fill-out the Service Request Form (fillable word file) and Email it to us.