Premade optional inducible lentiviral shRNA expression particles, knockdowning  firefly luciferase gene. Particles contains a GFP-Blasticidin dual selection marker. GFP fluorescent signal provide a convenient method to observe the virus tranduction under microscope, and the transuced cells can be sorted / selected via either flourescent signal or Blasticidin.

shRNA sequence was designed in firefly luciferase codon region, demonstrating greater than 75% knockdown levels for exogenous (trasnfected luciferase expression) in HEK293 cells. It also comes with its negative control shRNA particles (Cat#: LVP-Ctr-GB) that contains a negative shRNA-sequence. The negative control shRNA was designed having no homogous to any human or mouse transcripts, and should not knock down any human or mouse genes. Please see product manual for more details.

Amount: 200ul/tube at (1 x 10 ⁷ IFU/ml) in DMEM medium with 10% FBS,

This order is for: One tube of Cat#: LVP345-GB and one tube of  negative control, Cat#: LVP-Ctr-GB.