Premade optional inducible lentiviral shRNA expression particles, knockdowning human P53 gene (NM_000546). Particles contains a RFP-Puromycin dual selection marker. RFP fluorescent signal provide a convenient method to observe the virus tranduction under microscope, and the transuced cells can be sorted / selected via either flourescent signal or Puromycin.

shRNA sequence was designed in human P53 codon (ORF) region, demonstrating greater than 75% knockdown levels for both exogenous (trasnfected P53 expression) and endogenous P53 expression. It also comes with its negative control shRNA particles (Cat#: LVP-Ctr-RP) that contains a negative shRNA-sequence. The negative control shRNA was designed having no homogous to any human or mouse transcripts, and should not knock down any human or mouse genes. Please see product manual for more details.

Amount: 200ul/tube at (1 x 10 ⁷ IFU/ml) in DMEM medium with 10% FBS,

This order is for: One tube of Cat#: LVP343-RP and one tube of  negative control, Cat#: LVP-Ctr-RP.