Pre-made tetracycline regulator (TetR) lentiviral particles expressing sequence verified tetracycline regulator gene under suCMV promoter.  A blasticidin-RFP fusion marker under RSV promoter, allows to sort or select transduced cells for long term expression via RFP signal or via blasticidin antibiotic (Dual markers). TetR was expressed with N-term His-tag. See product manual (.pdf).

TetR  is used in tetracycline inducible expression. It binds to inducible promoter to repress the expression. And the expression is induced once tetracycline is added and binds to TetR, which release TetR from promoter. This is the blasticidin version of particles.

Amount:  200ul/per vial, at 1 x 10⁷ IFU/ml in DMEM medium with 10% FBS. 

 Cat#: LVP017-Bsd