The kit includes three parts: (1) the CAS9 expression lentivirus; (2) the target-specific gRNA lentivirus; (3) our proprietary repair donor template as the engineered knock-in cassette using engineered IDLD (“Integration Deficient Lentivector Donor”).
Our Lentivirus-based Donor template can provide large gene insertion (1kb to 5kb), with high-efficient, low off-target, less unwanted insertional mutagenesis in CRISPR editing. It greatly accelerates the gene-editing / therapy fields. The donor DNA provides the desired sequence insertion that flanked by the gene loci’s homology sequences, left homologous arm (LHA) and right homologous arm (RHA), for the genomic editing via HDR mechanism.
The Donor DNA can be single-strand or double-stranded DNA. Alternatively, it can be simply synthesized and delivered by lipid-based transfection or electroporation.