Cas9 (CAG, Neo) lentiviral particles

$295.00

SKU: LVP689 Categories: ,

Description

Lentivirus express human codon-optimized Cas9 endonuclease (Streptococcus pyogenes )  under  CAG promoter, with a proprietary NLS (Nuclear Localization Signal) at its N-terminal, for the best nuclear penetration. CAG promoter has strong promoter strength in some cell types where the CMV, or EF1a promoter strength is weak. 

It also contains a Neomycin (G418) antibiotic marker for the selection of the transduced cells. 

See Product Manual here (.pdf).

Application: used for CRISPR gene editing ( Note: used with a separate “gene-specific gRNA lentivirus” or ” gene-specific gRNA vector” for CRISPR editing )

Amount:  5 x 106 IFU/ml x 200ul in DMEM medium with 10% FBS, 10x of polybrene. 

Shipping and storage: overnight shipment in dry-ice package. Stored at -80oC.

Cat#: LVP689