Three shRNA lentiviectors (5.0 ug/each) and three pre-made shRNA expression lentivirus (0.5 ml/each). One negative control shRNA vector (5.0ug) and virus (0.5ml). Guaranteed knockdown (at least one shRNA has >75% knockdown level).
The shRNA was expressed under an optional Tetracycline inducible human H1 promoter. It can be used as constitutive expression of shRNA without any induction, or used as tetracycline inducible expression when the Tet-repressor protein is present in advance. The shRNA lentivector aslo contains a Puromycin marker under Rsv promoter, which allows to select the shRNA positive cell by Puromycin antibiotic selection. See shRNA cloning kit manual for more details about the shRNA lentivector
Order will be delivered within 7-10 business days.
Amounts: (1) Plasmid DNAs: 5 ug/each x 4 tubes; (2): pre-made shRNA virus: 0.5ml/each x 4 tubes. (vectors and viruses: three target specific shRNA and one negative control shRNA).
Note: please provide the target accession number during checkout in the “Comments” field, or email us your target sequence that you want the shRNA designed upon.