Pre-made GFP lentivial particles expressing codon optimized GFP under the CAG promoter. It contains a Puromycin antibiotic selection marker under Rsv promoter. See Product manual for details (.pdf).
The CAG promoter ( a hybrid of the CMV enhancer and chicken beta-actin promoter) has the highest expression level in embryonic stem (ES) cells.
- monitor lentivirus transduction efficiency for your cells of interest;
- stable cell line generation;
Amount: 1 x 107 IFU/ml x 200ul in DMEM medium with 10% FBS, 60ug/ml polybrene.