Lentivirus expresses codon optimized Green Fluorescent Protein (GFP) under the CAG promoter. A Puromycin (Puro) selection marker was express under Rsv promoter, which allows to select transduced fluorescent positive cells. The following scheme showed the lentivector core structure. See Product Manual (.pdf).
The CAG promoter ( a hybrid of the CMV enhancer and chicken beta-actin promoter) has the highest expression level in embryonic stem (ES) cells.
Amount: 1 x 107 IFU/ml x 200ul in DMEM medium with 10% FBS, 60ug/ml polybrene.