This particles was produced in our tetraycline inducible lentiviral system. It expresses codon optimized GFP under tetracycline inducible suCMV promoter. A RFP-Blasticidin fusion marker under the constitutive RSV promoter, allows to select transduced cells via blasticidin antibiotic or RFP signal sorting.
GFP is constitutively expressed in high level without any induction. But optionally, this particles can be used as Tetracycline inducible expression of GFP in the prescence of TetR protein (repressor). So it can be used alone for double fluorescent expression (GFP and RFP)particles, or used as the control particles to validate your tetracylcine inducible system when TetR is present. Please see Product manual for details.
Both GFP and RFP signal can be visualized under fluorescent microscope. The RFP is constitutively expressed and not affected by Tetracycline induction.
Amount: 200ul/per vial, at 1 x 107 IFU/ml in DMEM medium with 10% FBS with 10x polyberne.