GFP (mPGK, Puro) Lentiviral particles

$295.00

SKU: LVP1228 Categories: ,

Description

Lentivirus expresses codon optimized Green Fluorescent Protein (GFP) under murine phosphoglycerate kinase (mPGK) promoter.Puromycin gene under Rsv promoter allows to select transduced fluorescent positive cells. The following scheme represents its core expression cassette structure. See Product Manual (.pdf).

The mPGK promoter: this promoter contains five copies of the hexadeoxynucleotide, GGGCGG, a CCAAT sequence, but no TATA box. It can efficiently drive the expression of reporter or genes, and may preferably in some cell types.

Application:

  1. measure the mPGK promoter strength in your desired cell types;
  2. monitor lentivirus transduction efficiency for your cells of interest;
  3. create GFP expression stable cell line (via Puromycin selection);
  4. others: promoter and pathway research;

Amount: 1 x 107 IFU/ml x 200ul in in DMEM medium with 10% FBS and 10x of polybrene.

Cat#: LVP1228