Luciferase (firefly)-2A-GFP (CMV, Puro) Lentivirus

$350.00

Description

Pre-made Lentivirus express firefly  luciferase 3 gene under a modified CMV promoter. A GFP marker was co-expressed under the same promoter. Luciferase and GFP were bicistronically and constitutively expressed as two individual protein mediated by a 2A element. (optionally, you can turn it into Tet inducible expression by used together with its repressor, TetR). 

A Puromycin antibiotic marker was expressed under RSV promoter, allows to sort or select transduced cells via GFP signal or via Puromycin antibiotic (Dual selection).  GFP signal provides convenient, real-time monitoring of the lentivirus performance.  See Product Manual for details (.pdf).

Amount:  >200ul/per vial, at 1 x 107 IFU/ml (Premixed with Polybrene)

Cat#: LVP020