Pre-made RFP lentivial particles containing codon optimized Red Fluorescent Protein (RFP) marker under suCMV promoter. A Blasticidin antibiotic selection under Rsv promoter allows to select transduced fluorescent positive cells. The following scheme showed the lentivector core structure:
See Product Manual (.pdf).
- monitor lentivirus transduction efficiency for your cells of interest;
- create RFP expression stable cell line (via Blasticidin selection);
Amount: 1 x 107 IFU/ml x 200ul in DMEM medium with 10% FBS, 60ug/ml polybrene.