Pre-made lentiviral particles expressing a fusion target of (fusion of “RFP-LC3″). The codon sequence of human LC3 gene (NCBI accession ID: NM_022818.4, MAP1LC3A) was fusioned in frame with RFP at its N-terminal, expressed under a consitutive suCMV promoter, containing a puromycin antibiotic marker under the RSV promoter.
The protein encoded by this gene is one of the light chain subunit for MAP1A and MAP1B which are microtubule-associated proteins, mediated the physical interactions between microtubules and components of the cytoskeleton
Please see product manual for details.
Amount: 200ul/vial (1×107 IFU/ml), provided in DMEM medium with 10% FBS and 60ug/ml Polybrene (10x).