Pre-made lentiviral particles expressing a fusion target of (RFP-Luciferase /firefly). The codon optimized firefly luciferase was fusioned at its N-terminal with RFP protein, expressed under a constitutive suCMV promoter. A puromycin antibiotic selection marker is expressed under Rsv promoter.
It is a double signal marker for visualizing of Red fluorescent (RFP)signal and monitoring luciferase signal in vivo or in vitro from the same sample.
Please see Product Manual for details.
Amount: 200ul/vial (1×107 IFU/ml), provided in DMEM medium with 10% FBS and 60ug/ml Polybrene (10x), or provided in PBS solution for in vivo application.