Pre-made lentivirus express SV40 large T-antigen gene under an enhanced EF1a promoter. A Puromycin resistant gene was expressed under RSV promoter, which allows to select by Puromycin antibiotic, the transduced cells for long term expression.
SV40 large T-antigen causes cell immortalization and allow to produce high-titer lentivirus in your cell line. It was natively expressed without any tags. See Product Manual (.pdf).
Amount: 200ul/per vial at 1 x 107 IFU/ml