CRE reporter cells
LoxP-GFP/RFP color switch cell lines
Pre-made stable cell line, derived from HEK293 cells, constitutively expresses a “LoxP-GFP-Stop-LoxP-RFP-Stop” cassette under strong suCMV promoter. The expression cassette also contains an antibiotic marker (Puromycin, Blastididin, or Neomycin) under a RSV promoter. See the genomic integrated express cassette structure below.
The CRE reporting cell lines are used to monitor or confirm the efficiency of CRE recombination in vivo. It is a great method and easy tool to verify the performance of your CRE enzyme (your CRE expression plasmids, or pre-made CRE expression lentivirus, or purified CRE enzyme) in vivo conditions. It is also a control test to verify your CRE-loxP based system.
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