Brain Cell Report
About CaMKII Promoter:
Calcium / Calmodulin-dependent protein Kinase II (CaMKII) is a serine/threonine-specific protein kinase that is regulated by the Ca2+/calmodulin complex. CaMKII is involved in many signaling cascades that important to learning and memory. The CaMKII’s promoter is a neuron-specific promoter. Its expression restricted to excitatory neurons in the neocortex and hippocampus, also pyramidal neurons.
CaMKII promoter were used for transgene expression to obtain preferential gene expression in CNS for the excitatory neuron populations. It provides a great tool for the investigation in the diversity of cell types within a given brain structure.
Report Lentivirus for Brain Cells:
GenTarget developed a set of reporting lentivirus for selectively labeling Brain cancer cells. Those reporting lentivirus has a luminescent report or a fluorescent report under the promoter of human CAMKII gene (click to see its sequence) that specifically expressed in Brain tissue or cells. Those reporter lentivirus are best suitable for for the investigation in in vivo and in vitro physiological studies in which visualization of CaMKII- neuronal populations is required, as well as for the signal pathway research on CAMKII promoter regulation.
Those reporting lentivirus also constitutively express a fluorescent selection marker or an antibiotic selection marker under the RSV promoter (Rous Sarcoma Virus Promoter) which is a moderate to strong promoter in most cell types. This selection marker is used to select the lentivirus infected cells (to generate the stable cell lines) via antibiotic killing or fluorescent cell sorting. See the scheme below for lentivector core expression cassette.
The premade, ready-to-use reporter lentivirus provides a much easier tool to specifically labeling or reporting for human or mouse excitatory neurons in the neocortex and hippocampus, also pyramidal neurons in in vitro and in vivo via the luciferase signal or fluorescent signal.
see Product Manual for details (.pdf).