About CD14 Promoter:
CD14 is expressed mainly by Macrophages. The CD14 promoter contains a C/EBP and Sp1 site that are critical for tissue-specific regulation of CD14 gene expression. Monocytic Cell are the largest type of white blood cells and can differentiate into macrophages. Monocytes compose 2% to 10% of all white blood cells in the human body. Monocytes are generally identified in stained smears by the large kidney shaped or notched nucleus. These change into macrophages after entering into appropriate tissue spaces. CD14 has been viewed as a useful marker for Monocytes and Macrophage.
Lentivirus Labels Monocytes:
GenTarget developed a set of reporting lentivirus for specifically or preferably labeling Monocytes and Macrophage. Those reporting lentivirus has a luminescent report or a fluorescent report under the promoter of human CD14 gene (click to see its sequence) that specific expressed in Monocytes and Macrophage. Those reporter lentivirus are best suitable for infecting the human or mouse Monocytes and Macrophage, as well as for the signal pathway research on CD14 promoter regulation.
Those reporting lentivirus also constitutively express a fluorescent selection marker or an antibiotic selection marker under the RSV promoter (Rous Sarcoma Virus Promoter) which is a moderate to strong promoter in most cell types. This selection marker is used to select the lentivirus infected cells (to generate the stable cell lines) via antibiotic killing or fluorescent cell sorting. See the scheme below for lentivector core expression cassette.
see Product Manual for details (.pdf).