Muscle Cell Reporter

Myoglobin (MB) Promoter: Myoglobin is an iron- and oxygen-binding protein found only in the muscle tissue. (It is related to hemoglobin, which is the iron- and oxygen-binding protein in blood, specifically in the red blood cells.). Myoglobin binds molecular oxygen to facilitate its diffusion from the vascular system to the muscle mitochondria. The myoglobin promoter can drive the expression of a reporter (or a transgene) in multinucleated myotubes but not in  mononucleated and undifferentiated cells.

GenTarget developed a set of reporting lentivirus for specifically labeling muscle cells, specially in cardiac and skeletal myocytes. Those reporting lentivirus has a luminescent report or a fluorescent report under the promoter of human Myoglobin gene (click to see its sequence) that specifically expressed in muscle, predominantly in cardiac and skeletal myocytes. Those reporter lentivirus are best suitable for infecting the human or mouse cardiac and skeletal myocytes, as well as for the signal pathway research on Myoglobin promoter regulation.

 Those reporting lentivirus also constitutively express a fluorescent selection marker or an antibiotic selection marker under the RSV promoter (Rous Sarcoma Virus Promoter) which is a moderate to strong promoter in most cell types. This selection marker is used to select the lentivirus infected cells (to generate the stable cell lines) via antibiotic killing or fluorescent cell sorting. It also  provides internal reference for virus transduction efficiency when a fluorescent marker is under the RSV promoter (wherever the RSV promoter is active in assay cell type). See the scheme below for lentivector’s core expression cassette.

The premade, ready-to-use reporter lentivirus provides a much easier tool to specifically labeling or reporting for human or mouse cardiac and skeletal myocytes in vitro and in vivo via the luciferase signal or fluorescent signal.

The premade, ready-to-use reporter lentivirus provides in two formats: 200ul in DMEM medium with 10% FBS and 10x polybrene, or 200ul concentrated virus in PBS solution. See each product below, or click to see Product Manual for details (.pdf).