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Home / Cell Immortalization / SV40 Large T antigen

SV40 Large T antigen

About SV40 large T antigen:

SV40 large T antigen (click to see its coding sequence), [Simian Vacuolating Virus 40 Large T-Ag ] is a hexamer protein involved in viral genome replication and regulation of the host cell cycle. It is  used as a model protein to study nuclear localization signals and viral replication. It also widely used for cell immortalization for variety of cell types.

SV40 large T antigen Expression Lentivirus:

GenTarget offers  premade expression lentivirus for SV40 large T-antigen, driven by either the super strong suCMV promoter or an enhanced EF1a promoter. Each lentivirus contains an antibiotic selection marker: Neomycin (Neo), Puromycin (Puro), Blasticidin (Bsd), Zeocin (Zeo), Hygromycin (Hygro), or contains a fluorescent-antibiotic fusion dual marker: RFP-Bsd, RFP-Puro, GFP-Bsd, GFP-Puro,  best for selecting, sorting and monitoring the immortalized cells.  See the lentivector expression scheme below:

SV40-T-antigen expression lentivector

Please see Product Manual for details.

Lentivirus are provided as  in two formats:

  1. Crude Lentivirus: 200ul /vial in DMEM containing 10 x Polybrene;
  2. Concentrated lentivirus: 200ul PBS solution which is better for hard-to-transduced cell types or for serum-free culture.
NameSKUPriceBuy
SV40 large T-antigen (CMV, Bsd) lentivirusLVP016-Bsd$350.00
SV40 large T-antigen (CMV, Bsd) lentivirus in PBSLVP016-Bsd-PBS$650.00
SV40 large T-antigen (CMV, GFP-Bsd) lentivirusLVP016-GB$350.00
SV40 large T-antigen (CMV, GFP-Bsd) lentivirus in PBSLVP016-GB-PBS$650.00
SV40 large T-antigen (CMV, GFP-Puro) lentivirusLVP016-GP$350.00
SV40 large T-antigen (CMV, GFP-Puro) lentivirus in PBSLVP016-GP-PBS$650.00
SV40 large T-antigen (CMV, Hygro) lentivirusLVP016-Hygro$350.00
SV40 large T-antigen (CMV, Hygro) lentivirus in PBSLVP016-Hygro-PBS$650.00
SV40 large T-antigen (CMV, Neo) lentivirusLVP016-Neo$350.00
SV40 large T-antigen (CMV, Neo) lentivirus in PBSLVP016-Neo-PBS$650.00
SV40 large T-antigen (CMV, No selection) lentivirusLVP016$350.00
SV40 large T-antigen (CMV, No Selection) lentivirus in PBSLVP016-PBS$650.00
SV40 large T-antigen (CMV, Puro) lentivirusLVP016-Puro$350.00
SV40 large T-antigen (CMV, Puro) lentivirus in PBSLVP016-Puro-PBS$650.00
SV40 large T-antigen (CMV, RFP-Bsd) lentivirusLVP016-RB$350.00
SV40 large T-antigen (CMV, RFP-Bsd) lentivirus in PBSLVP016-RB-PBS$650.00
SV40 large T-antigen (CMV, RFP-Puro) lentivirusLVP016-RP$350.00
SV40 large T-antigen (CMV, RFP-Puro) lentivirus in PBSLVP016-RP-PBS$650.00
SV40 large T-antigen (CMV, Zeo) lentivirusLVP016-Zeo$350.00
SV40 large T-antigen (CMV, Zeo) lentivirus in PBSLVP016-Zeo-PBS$650.00
SV40 large T-antigen (EF1a, Bsd) lentivirusLVP557-Bsd$350.00
SV40 large T-antigen (EF1a, Bsd) lentivirus in PBSLVP557-Bsd-PBS$650.00
SV40 large T-antigen (EF1a, GFP-Bsd) lentivirusLVP557-GB$350.00
SV40 large T-antigen (EF1a, GFP-Bsd) lentivirus in PBSLVP557-GB-PBS$650.00
SV40 large T-antigen (EF1a, GFP-Puro) lentivirusLVP557-GP$350.00
SV40 large T-antigen (EF1a, GFP-Puro) lentivirus in PBSLVP557-GP-PBS$650.00
SV40 large T-antigen (EF1a, Hygro) lentivirusLVP557-Hygro$350.00
SV40 large T-antigen (EF1a, Hygro) lentivirus in PBSLVP557-hygro-PBS$650.00
SV40 large T-antigen (EF1a, Neo) lentivirusLVP557-Neo$350.00
SV40 large T-antigen (EF1a, Neo) lentivirus in PBSLVP557-Neo-PBS$650.00
SV40 large T-antigen (EF1a, No selection) lentivirusLVP557$350.00
SV40 large T-antigen (EF1a, No Selection) lentivirus in PBSLVP557-PBS$650.00
SV40 large T-antigen (EF1a, Puro) lentivirusLVP557-Puro$350.00
SV40 large T-antigen (EF1a, Puro) lentivirus in PBSLVP557-Puro-PBS$650.00
SV40 large T-antigen (EF1a, RFP-Bsd) lentivirusLVP557-RB$350.00
SV40 large T-antigen (EF1a, RFP-Bsd) lentivirus in PBSLVP557-RB-PBS$650.00
SV40 large T-antigen (EF1a, RFP-puro) lentivirusLVP557-RP$350.00
SV40 large T-antigen (EF1a, RFP-puro) lentivirus in PBSLVP557-RP-PBS$650.00
SV40 large T-antigen (EF1a, Zeo) lentivirusLVP557-Zeo$350.00
SV40 large T-antigen (EF1a, Zeo) lentivirus in PBSLVP557-Zeo-PBS$650.00

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