Glucocorticoid Pathway

Glucocorticoid Pathway: The glucocorticoid (GC) a class of steroid hormones. GC mediated signaling transduction is an important class of transcription factors in response to a specific hormone signal. The binding of GC to its receptor, glucocorticoid receptor (GR) via the glucocorticoid response element (G-RE) sequence, alters the target gene expression that involves in anti-inflammatory, the tumor cell growth and apoptosis, cardiovascular, metabolic, immunologic, and homeostatic functions, other cytotoxicity, in almost every vertebrate animal cell.

GenTarget developed a set of glucocorticoid pathway reporter lentivirus to monitor glucocorticoid receptor-induced signal transduction pathways. Those reporting lentivirus has a luminescent report (Luciferase, Renilla Luc) or a fluorescent report (GFP, RFP) or a  secreted SEAP report, under a minimal CMV promoter (mCMV) that embedded with optimized tandem repeats of glucocorticoid response element (G-RE) sequence motif (5′- AGAACAtttTGTTCT; GGTACAtttTGTTCT). When glucocorticoids are present and bind to G-RE sequence, the downstream reporter is expressed as the result of activation for the minimal promoter.

The luciferase signal can be measured via Luciferase assay. The fluorescent reporter can be detected via its fluorescent signal. The SEAP secreted into cell culture supernatant, which allows to determine reporter activity without disturbing the cells, does not require the preparation of cell lysates and can be used for kinetic studies.

Those reporting lentivirus also contains a constitutively expressed fluorescent selection marker or an antibiotic selection marker under the RSV promoter, which makes it easier to select the stably infected signal reporting cells (to generate pathway specific sensor cell lines), or provides internal reference for virus transduction efficiency when a fluorescent marker is under the RSV promoter. See core lentivector scheme below.

The premade, ready-to-use reporter lentivirus provides a much easier tool to monitor the activity of glucocorticoid-mediated signaling pathways in virtually any mammalian cell type. It also allows to generate your own reporting cell line in your desired cell type for study or screen of pathway specific gene-knockdown, over-expression, or chemical / drug/protein treatment (such as Glucocorticoids / glucocorticosteroid) in the cell based assay.

Click the Product Manual (pdf) for all available products for this pathway.