Prostate Cell Reporter

PSA  Promoter: Prostate-specific antigen (PSA) is secreted by the epithelial cells of the prostate gland. PSA is expressed at very low level in healthy prostate, but is often elevated in the presence of prostate cancer or other prostate disorders. PSA is a serum marker for prostate cancer.

PSA promoter contains the androgen response elements (AREs). PSA expression is tightly regulated by androgen. Upon binding to androgen, androgen receptor (AR) translocates into the nucleus and binds to the PSA promoter’s AREs, where it interacts with other transcription factors and activates PSA gene transcription. PSA promoter activity is known to be highly prostate tissue or cell-specific.

GenTarget developed a set of reporting lentivirus for selectively labeling prostate cancer cells. Those reporting lentivirus has a luminescent report or a fluorescent report under the promoter of human PSA  gene (click to see its sequence) that specifically expressed in prostate tissue or cells. Those reporter lentivirus are best suitable for infecting the human or mouse prostate cells or prostate cancer, as well as for the signal pathway research on PSA  promoter regulation.

Those reporting lentivirus also constitutively express a fluorescent selection marker or an antibiotic selection marker under the RSV promoter (Rous Sarcoma Virus Promoter) which is a moderate to strong promoter in most cell types. This selection marker is used to select the lentivirus infected cells (to generate the stable cell lines) via antibiotic killing or fluorescent cell sorting. It also  services as internal reference for virus transduction efficiency when a fluorescent marker is under the RSV promoter (wherever the RSV promoter is active in assay cell type). See the scheme below for lentivector’s core expression cassette.

The premade, ready-to-use reporter lentivirus provides a much easier tool to specifically labeling or reporting for human or mouse prostate cells in vitro and in vivo via the luciferase signal or fluorescent signal.

The premade, ready-to-use reporter lentivirus provides in two formats: 200ul in DMEM medium with 10% FBS and 10x polybrene, or 200ul concentrated virus in PBS solution. See each product below, or click to see Product Manual for details (.pdf).